It is a specific analytical test for aldehydes and ketones. carbonyls react with 2,4-Dinitrophenylhydrazine forming phenylhydrazones which precipitate yellow. The appearance of a precipitate is an indicator of the presence of carbonyls in the medium.
The mechanism of the reaction is analogous to that of imine formation.
2,4-Dinitrophenylhydrazine assay
- Details
- Germán Fernández
- THEORY OF ALDEHYDES AND KETONES
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