The synthesis of the dipeptide Glycine-Alanine (Gly-Ala) takes place through the formation of the amide bond (peptide) between the acid group of Glycine and the amino group of alanine. Therefore, it is necessary to protect the amino group of Glycine and the acid of alanine.
Stage 1. Protection of the amino group of Glycine.
Stage 2. Protection of the acid group of Alanine
Step 3. Formation of the peptide bond, mediated by dicyclohexylcarbodiimide (DCC)
Step 4. Group checkout
The mechanism of stage 3 takes place in the following steps:
