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Myristic acid reaction site in a compound with 2 hydroxyl groups 7 months 2 weeks ago #14386

Dear all,Portimine-A is a compound containing two hydroxyl groups (ChemSpider ID 30771030). When I semi-synthesized portimine-A esters by using DCC (dehydrating agent), myristic acid, and portimine-A, I only observe one peak (peak #1 at 10.7 min) by LC-MS/HRMS analysis, suggesting that only one hydroxyl had undergone acylation.

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[/img]When the same reaction is carried out with DMAP as catalyst (Steglich reaction), I observed two peaks at different retention times (peak #1 at 10.7 min and peak #2 at 11.4 min). Both peaks had the same elemental composition, corresponding to C14:0-portimine-A (C37H57NO6). I suppose that these two peaks correspond to the 5-O-myristoyl and 13-O-myristoyl esters of portimine-A. I also observed another peak with an elemental composition matching that of the doubly acylated form of portimine-A (5,13-di-O-myristoyl ester of portimine-A).I would like to know if the peak #1 observed by LC-MS/HRMS corresponds to the 5-O-myristoyl or the 13-O-myristoyl esters of portimine-A. As my background is in analytical chemistry, with limited knowledge in organic chemistry, I was wondering if you think it is possible to determine this based on the reactivity of the two hydroxyl groups or other information.

Kind regards,
Vincent

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